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ProductsBack/MX1 antibody [2G12]
WB analysis of various samples using GTX84067 MX1 antibody [2G12]. Loading : 10 ug per lane Dilution : 1:200
WB analysis of various samples using GTX84067 MX1 antibody [2G12]. Loading : 10 ug per lane Dilution : 1:200
WB analysis of various samples using GTX84067 MX1 antibody [2G12]. Loading : 10 ug per lane Dilution : 1:200

MX1 antibody [2G12]

GTX84067
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
TargetMX1
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Overview

  • Supplier
    GeneTex
  • Product Name
    MX1 antibody [2G12] - Overexpression Validated
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000-1:2000. ICC/IF: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    2G12
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4599
  • Target name
    MX1
  • Target description
    MX dynamin like GTPase 1
  • Target synonyms
    IFI78; IFI-78K; interferon-induced GTP-binding protein Mx1; interferon-induced protein p78; interferon-inducible protein p78; interferon-regulated resistance GTP-binding protein MxA; lncMX1-215; MX; MxA; myxoma resistance protein 1; myxovirus (influenza virus) resistance 1, interferon-inducible protein p78
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP20591
  • Protein Name
    Interferon-induced GTP-binding protein Mx1
  • Scientific Description
    May regulate the calcium channel activity of TRPCs. Shows activity against influenza virus and VSV, a rhabdovirus.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MX1 using anti-MX1 antibody (A00849-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MX1 antigen affinity purified polyclonal antibody (Catalog # A00849-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MX1 at approximately 76 kDa. The expected band size for MX1 is at 76 kDa.
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Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular as well as lymphoid cells.
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