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Mybpc3 Polyclonal Antibody

CAC09252
Biomatik
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetMYBPC3
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Overview

  • Supplier
    Biomatik
  • Product Name
    MYBPC3 Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA, WB; Recommended dilution: WB:1:500-1:5000
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4607
  • Target name
    MYBPC3
  • Target description
    myosin binding protein C3
  • Target synonyms
    CMD1MM, CMH4, FHC, LVNC10, MYBP-C, cMyBP-C, myosin-binding protein C, cardiac-type, myosin binding protein C, cardiac
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14896
  • Protein Name
    Myosin-binding protein C, cardiac-type
  • Scientific Description
    The Mybpc3 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB.
  • Reactivity Supplier
    Human, Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MYBPC3 using anti-MYBPC3 antibody (A01078-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYBPC3 antigen affinity purified polyclonal antibody (Catalog # A01078-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MYBPC3 at approximately 160KD. The expected band size for MYBPC3 is at 147KD.
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