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Western blot analysis of extracts of various cell lines using MYCBP Polyclonal Antibody at dilution of 1:3000.
Western blot analysis of extracts of various cell lines using MYCBP Polyclonal Antibody at dilution of 1:3000.
Western blot analysis of extracts of various cell lines using MYCBP Polyclonal Antibody at dilution of 1:3000.

MYCBP Polyclonal Antibody

E-AB-65487
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    MYCBP Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    The protein encoded by this gene binds to the N-terminus of the oncogenic protein C-MYC, enhancing the ability of C-MYC to activate E box-dependent transcription. The encoded protein is normally found in the cytoplasm, but it translocates to the nucleus during S phase of the cell cycle and associates with C-MYC. This protein may be involved in spermatogenesis. This gene can be silenced by microRNA-22. Two transcript variants, one protein-coding and the other probably not protein-coding, have been found for this gene.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MYCBP using anti-MYCBP antibody (A06216-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human 293T whole cell lysates; Lane 3: rat lung tissue lysates; Lane 4: rat NRK whole cell lysates; Lane 5: mouse lung tissue lysates; Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYCBP antigen affinity purified polyclonal antibody (Catalog # A06216-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MYCBP at approximately 12 kDa. The expected band size for MYCBP is at 12 kDa.
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