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WB analysis of COS7 cell lysates using GTX87110 MYF5 antibody. The lane on the right is blocked with the synthesized peptide.
WB analysis of COS7 cell lysates using GTX87110 MYF5 antibody. The lane on the right is blocked with the synthesized peptide.
WB analysis of COS7 cell lysates using GTX87110 MYF5 antibody. The lane on the right is blocked with the synthesized peptide.

MYF5 antibody

GTX87110
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Monkey, Mouse
TargetMYF5
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Overview

  • Supplier
    GeneTex
  • Product Name
    MYF5 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500~1:1000. ICC/IF: 1:100~1:500. IHC-P: 1:50~1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4617
  • Target name
    MYF5
  • Target description
    myogenic factor 5
  • Target synonyms
    EORVA, bHLHc2, myogenic factor 5, class C basic helix-loop-helix protein 2, myf-5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP13349
  • Protein Name
    Myogenic factor 5
  • Reactivity
    Human, Monkey, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

References

  • Unique Features of River Lamprey (Lampetra fluviatilis) Myogenesis.
    Read this paper
  • Trichinella spiralis muscle larvae excretory-secretory products induce changes in cytoskeletal and myogenic transcription factors in primary myoblast cultures. Hernandez-Ancheyta L et al., 2018 Mar, Int J Parasitol
    Read this paper
  • p38 MAPK activation and H3K4 trimethylation is decreased by lactate in vitro and high intensity resistance training in human skeletal muscle. Willkomm L et al., 2017, PLoS One
    Read this paper

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Figure 1. Western blot analysis of Myf5 using anti-Myf5 antibody (A04040-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SW620 cell lysates, Lane 2: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Myf5 antigen affinity purified polyclonal antibody (Catalog # A04040-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Myf5 at approximately 28KD. The expected band size for Myf5 is at 28KD.
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