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WB analysis of mouse skeletal and muscle tissue using GTX77867 MYF5 antibody at 1:1000. Lane1: Mouse heart lysate Lane2: Mouse skeletal muscle lysate
WB analysis of mouse skeletal and muscle tissue using GTX77867 MYF5 antibody at 1:1000. Lane1: Mouse heart lysate Lane2: Mouse skeletal muscle lysate
WB analysis of mouse skeletal and muscle tissue using GTX77867 MYF5 antibody at 1:1000. Lane1: Mouse heart lysate Lane2: Mouse skeletal muscle lysate

MYF5 antibody, N-term

GTX77867
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetMYF5
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Overview

  • Supplier
    GeneTex
  • Product Name
    MYF5 antibody, N-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.2-2.5 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4617
  • Target name
    MYF5
  • Target description
    myogenic factor 5
  • Target synonyms
    EORVA, bHLHc2, myogenic factor 5, class C basic helix-loop-helix protein 2, myf-5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP13349
  • Protein Name
    Myogenic factor 5
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Myf5 using anti-Myf5 antibody (A04040-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SW620 cell lysates, Lane 2: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Myf5 antigen affinity purified polyclonal antibody (Catalog # A04040-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Myf5 at approximately 28KD. The expected band size for Myf5 is at 28KD.
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