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WB analysis of 293 cell lysates using GTX87578 MYH14 antibody. The lane on the right is blocked with the synthesized peptide.
WB analysis of 293 cell lysates using GTX87578 MYH14 antibody. The lane on the right is blocked with the synthesized peptide.
WB analysis of 293 cell lysates using GTX87578 MYH14 antibody. The lane on the right is blocked with the synthesized peptide.

MYH14 antibody

GTX87578
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetMYH14
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Overview

  • Supplier
    GeneTex
  • Product Name
    MYH14 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500~1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID79784
  • Target name
    MYH14
  • Target description
    myosin heavy chain 14
  • Target synonyms
    DFNA4; DFNA4A; FP17425; MHC16; MYH14 variant protein; MYH17; myosin; myosin heavy chain, non-muscle IIc; myosin, heavy chain 14, non-muscle; myosin, heavy polypeptide 14; myosin-14; NMHC II-C; NMHC-II-C; non-muscle myosin heavy chain IIc; nonmuscle myosin heavy chain II-C; PNMHH
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ7Z406
  • Protein Name
    Myosin-14
  • Scientific Description
    This gene encodes a member of the myosin superfamily. The protein represents a conventional non-muscle myosin; it should not be confused with the unconventional myosin-14 (MYO14). Myosins are actin-dependent motor proteins with diverse functions including regulation of cytokinesis, cell motility, and cell polarity. Mutations in this gene result in one form of autosomal dominant hearing impairment. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MYH14 using anti-MYH14 antibody (A04528-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: rat lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYH14 antigen affinity purified polyclonal antibody (Catalog # A04528-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MYH14 at approximately 250 kDa. The expected band size for MYH14 is at 228 kDa.
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