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Western Blot analysis of 293 cells using MYH14 Polyclonal Antibody
Western Blot analysis of 293 cells using MYH14 Polyclonal Antibody
Western Blot analysis of 293 cells using MYH14 Polyclonal Antibody

MYH14 Antibody

CSB-PA003343
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetMYH14
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Overview

  • Supplier
    Cusabio
  • Product Name
    MYH14 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID79784
  • Target name
    MYH14
  • Target description
    myosin heavy chain 14
  • Target synonyms
    DFNA4, DFNA4A, FP17425, MHC16, MYH17, NMHC II-C, NMHC-II-C, PNMHH, myosin, myosin-14, MYH14 variant protein, myosin heavy chain, non-muscle IIc, myosin, heavy chain 14, non-muscle, myosin, heavy polypeptide 14, non-muscle myosin heavy chain IIc, nonmuscle myosin heavy chain II-C
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ7Z406
  • Protein Name
    Myosin-14
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MYH14 using anti-MYH14 antibody (A04528-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: rat lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYH14 antigen affinity purified polyclonal antibody (Catalog # A04528-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MYH14 at approximately 250 kDa. The expected band size for MYH14 is at 228 kDa.
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