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Anti-Myosin 14 Antibody

102-26719
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetMYH14
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-Myosin 14 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID79784
  • Target name
    MYH14
  • Target description
    myosin heavy chain 14
  • Target synonyms
    DFNA4, DFNA4A, FP17425, MHC16, MYH17, NMHC II-C, NMHC-II-C, PNMHH, myosin, myosin-14, MYH14 variant protein, myosin heavy chain, non-muscle IIc, myosin, heavy chain 14, non-muscle, myosin, heavy polypeptide 14, non-muscle myosin heavy chain IIc, nonmuscle myosin heavy chain II-C
  • Host
    Rabbit
  • Protein IDQ7Z406
  • Protein Name
    Myosin-14
  • Scientific Description
    Rabbit Anti-Myosin 14 Antibody, 100 microl
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MYH14 using anti-MYH14 antibody (A04528-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: rat lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYH14 antigen affinity purified polyclonal antibody (Catalog # A04528-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MYH14 at approximately 250 kDa. The expected band size for MYH14 is at 228 kDa.
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