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MYH6/MYH7 Antibody

PACO07425
Assay Genie
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMYH6
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Overview

  • Supplier
    Assay Genie
  • Product Name
    MYH6/MYH7 Antibody
  • Delivery Days Customer
    9
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    IHC, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    Not applicable
  • Conjugate
    Unconjugated
  • Gene ID4624
  • Target name
    MYH6
  • Target description
    myosin heavy chain 6
  • Target synonyms
    ASD3, CMD1EE, CMH14, MYHC, MYHCA, SSS3, alpha-MHC, myosin-6, myHC-alpha, myosin, heavy polypeptide 6, cardiac muscle, alpha (cardiomyopathy, hypertrophic 1)
  • Host
    Rabbit
  • Protein IDP12883
  • Protein Name
    Myosin-7
  • Scientific Description
    MYH6/MYH7 Antibody is a premium polyclonal that offers outstanding performance and reliability for demanding research applications. Rigorously validated for IHC, ELISA, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Human, Mouse, Rat samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 50ug format to meet your experimental needs. For optimal performance, store at -20°C or -80°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Human, Mouse, Rat
  • Reactivity Supplier
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of MYH6 using anti-MYH6 antibody (A02808-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYH6 antigen affinity purified polyclonal antibody (Catalog # A02808-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MYH6 at approximately 224 kDa. The expected band size for MYH6 is at 224 kDa.
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IHC image of CSB-PA015299HA01HU diluted at 1:100 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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