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myosin light chain 3 Recombinant Antibody

BSM-60346R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetMYL3
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    myosin light chain 3 Recombinant Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500), IF(), Flow-Cyt(1ug/Test)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    F12A11
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4634
  • Target name
    MYL3
  • Target description
    myosin light chain 3
  • Target synonyms
    cardiac myosin light chain 1; CMH8; CMLC1; MLC1SB; MLC1V; MLC-lV/sb; myosin light chain 3; myosin, light chain 3, alkali; ventricular, skeletal, slow; myosin, light polypeptide 3, alkali; ventricular, skeletal, slow; ventricular myosin alkali light chain; ventricular myosin light chain 1; ventricular/slow twitch myosin alkali light chain; VLC1; VLCl
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08590
  • Protein Name
    Myosin light chain 3
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MYL3 using anti-MYL3 antibody (A04730-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human H9C2(2-1) whole cell lysates, Lane 4: mouse heart tissue lysates, Lane 5: mouse skeletal muscle tissue lysates, Lane 6: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYL3 antigen affinity purified polyclonal antibody (Catalog # A04730-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MYL3 at approximately 25 kDa. The expected band size for MYL3 is at 22 kDa.
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