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Anti-MYLK [ARC0842] Monoclonal Antibody

CAB3835
Assay Genie
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetMYLK
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Overview

  • Supplier
    Assay Genie
  • Product Name
    Anti-MYLK [ARC0842] Monoclonal Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    ARC0842
  • Conjugate
    Unconjugated
  • Gene ID4638
  • Target name
    MYLK
  • Target description
    myosin light chain kinase
  • Target synonyms
    AAT7, KRP, MLCK, MLCK1, MLCK108, MLCK210, MMIHS, MMIHS1, MSTP083, MYLK-L, MYLK1, smMLCK, myosin light chain kinase, smooth muscle, kinase-related protein, myosin, light polypeptide kinase, smooth muscle myosin light chain kinase, telokin
  • Host
    Rabbit
  • Protein IDQ15746
  • Protein Name
    Myosin light chain kinase, smooth muscle
  • Scientific Description
    MYLK Monoclonal Antibody is a premium monoclonal that offers outstanding performance and reliability for demanding research applications. Rigorously validated for WB, ELISA, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Human,Rat samples, providing researchers with confidence in cross-species compatibility. Derived from the well-characterized ARC0842 clone, ensuring batch-to-batch consistency and reproducibility. Conveniently packaged in 100microL format to meet your experimental needs. For optimal performance, store at -20°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Human
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MYLK using anti-MYLK antibody (A01697-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SGC-7901 whole cell lysates, Lane 2: rat spleen tissue lysates, Lane 3: mouse spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYLK antigen affinity purified polyclonal antibody (Catalog # A01697-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MYLK at approximately 250KD. The expected band size for MYLK is at 211KD.
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