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ProductsBack/MYLK Monoclonal Antibody

MYLK Monoclonal Antibody

CAB3835
Assay Genie
TargetMYLK
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    MYLK Rabbit mAb
  • Delivery Days Customer
    9
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID4638
  • Target name
    MYLK
  • Target description
    myosin light chain kinase
  • Target synonyms
    AAT7; kinase-related protein; KRP; MLCK; MLCK1; MLCK108; MLCK210; MMIHS; MMIHS1; MSTP083; MYLK1; myosin light chain kinase, smooth muscle; myosin, light polypeptide kinase; smMLCK; smooth muscle myosin light chain kinase; telokin
  • Host
    Rabbit
  • Protein IDQ15746
  • Protein Name
    Myosin light chain kinase, smooth muscle
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MLCK Polyclonal Antibody(bs-4705R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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Figure 1. Western blot analysis of MYLK using anti-MYLK antibody (A01697-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SGC-7901 whole cell lysates, Lane 2: rat spleen tissue lysates, Lane 3: mouse spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYLK antigen affinity purified polyclonal antibody (Catalog # A01697-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MYLK at approximately 250KD. The expected band size for MYLK is at 211KD.
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