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Immunohistochemistry of paraffin-embedded human lymph node tissue using CSB-PA015344LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human lymph node tissue using CSB-PA015344LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human lymph node tissue using CSB-PA015344LA01HU at dilution of 1:100

MYO1G Antibody

CSB-PA015344LA01HU
Cusabio
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMYO1G
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Overview

  • Supplier
    Cusabio
  • Product Name
    MYO1G Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID64005
  • Target name
    MYO1G
  • Target description
    myosin IG
  • Target synonyms
    HA2, HLA-HA2, MHAG, unconventional myosin-Ig, minor histocompatibility antigen HA-2, myosin-Ig
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDB0I1T2
  • Protein Name
    Unconventional myosin-Ig
  • Scientific Description
    Unconventional myosin required during immune response for detection of rare antigen-presenting cells by regulating T-cell migration. Unconventional myosins are actin-based motor molecules with ATPase activity and serve in intracellular movements. Acts as a regulator of T-cell migration by generating membrane tension, enforcing cell-intrinsic meandering search, thereby enhancing detection of rare antigens during lymph-node surveillance, enabling pathogen eradication. Also required in B-cells, where it regulates different membrane/cytoskeleton-dependent processes. Involved in Fc-gamma receptor (Fc-gamma-R) phagocytosis.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MYO1G using anti-MYO1G antibody (A08448-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYO1G antigen affinity purified polyclonal antibody (Catalog # A08448-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MYO1G at approximately 116 kDa. The expected band size for MYO1G is at 116 kDa.
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Immunohistochemistry analysis in human bone marrow and cerebral cortex tissues using Anti-MYO1G antibody. Corresponding MYO1G RNA-seq data are presented for the same tissues.
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