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Western Blot analysis of Jurkat cells using Myosin VI Polyclonal Antibody
Western Blot analysis of Jurkat cells using Myosin VI Polyclonal Antibody
Western Blot analysis of Jurkat cells using Myosin VI Polyclonal Antibody

MYO6 Antibody

CSB-PA004696
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMYO6
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Overview

  • Supplier
    Cusabio
  • Product Name
    MYO6 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4646
  • Target name
    MYO6
  • Target description
    myosin VI
  • Target synonyms
    DFNA22, DFNB37, unconventional myosin-VI, unconventional myosin-6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UM54
  • Protein Name
    Unconventional myosin-VI
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of MYO6 using anti-MYO6 antibody (A02627-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYO6 antigen affinity purified polyclonal antibody (Catalog # A02627-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MYO6 at approximately 150 kDa. The expected band size for MYO6 is at 150 kDa.
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