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Western blot All lanes: MYOT antibody at 2microg/ml Lane 1: 293T whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: Mouse skeletal muscle tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 56, 36 kDa Observed band size: 56 kDa
Western blot All lanes: MYOT antibody at 2microg/ml Lane 1: 293T whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: Mouse skeletal muscle tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 56, 36 kDa Observed band size: 56 kDa
Western blot All lanes: MYOT antibody at 2microg/ml Lane 1: 293T whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: Mouse skeletal muscle tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 56, 36 kDa Observed band size: 56 kDa

MYOT Antibody

CSB-PA015364DSR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetMYOT
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Overview

  • Supplier
    Cusabio
  • Product Name
    MYOT Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9499
  • Target name
    MYOT
  • Target description
    myotilin
  • Target synonyms
    LGMD1, LGMD1A, MFM3, TTID, TTOD, myotilin, 57 kDa cytoskeletal protein, myofibrillar titin-like Ig domains protein, titin immunoglobulin domain protein (myotilin)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UBF9
  • Protein Name
    Myotilin
  • Scientific Description
    Component of a complex of multiple actin cross-linking proteins. Involved in the control of myofibril assembly and stability at the Z lines in muscle cells.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MYOT using anti-MYOT antibody (A04663-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat skeletal muscle tissue lysates, Lane 6: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYOT antigen affinity purified polyclonal antibody (Catalog # A04663-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MYOT at approximately 55 kDa. The expected band size for MYOT is at 55 kDa.
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Immunohistochemistry analysis in human skeletal muscle and skin tissues using Anti-MYOT antibody. Corresponding MYOT RNA-seq data are presented for the same tissues.
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