![Wild-type (WT) and N-Cadherin knockout (KO) 293T cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N2C1], Internal (GTX112733) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Wild-type (WT) and N-Cadherin knockout (KO) 293T cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N2C1], Internal (GTX112733) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX112733/GTX112733_40394_20180810_WB_KO_watermark_w_23060500_602.webp)
Wild-type (WT) and N-Cadherin knockout (KO) 293T cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N2C1], Internal (GTX112733) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
N-Cadherin antibody [N2C1], Internal
GTX112733
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCDH2
Overview
- SupplierGeneTex
- Product NameN-Cadherin antibody [N2C1], Internal
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.53 mg/ml
- ConjugateUnconjugated
- Gene ID1000
- Target nameCDH2
- Target descriptioncadherin 2
- Target synonymsACOGS, ADHD8, ARVD14, CD325, CDHN, CDw325, NCAD, cadherin-2, N-cadherin 1, cadherin 2, type 1, N-cadherin (neuronal), calcium-dependent adhesion protein, neuronal, neural cadherin
- HostRabbit
- IsotypeIgG
- Protein IDP19022
- Protein NameCadherin-2
- Scientific DescriptionThis gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Şen Ö, Emanet M, Mazzuferi M, et al. Microglia Polarization and Antiglioma Effects Fostered by Dual Cell Membrane-Coated Doxorubicin-Loaded Hexagonal Boron Nitride Nanoflakes. ACS Appl Mater Interfaces. 2023,15(50):58260-58273. doi: 10.1021/acsami.3c17097Read this paper
- Liang ZL, Wu DD, Yao Y, et al. Epiproteome profiling of cadmium-transformed human bronchial epithelial cells by quantitative histone post-translational modification-enzyme-linked immunosorbent assay. J Appl Toxicol. 2018,38(6):888-895. doi: 10.1002/jat.3597Read this paper
- Chen YJ, Wang WH, Wu WY, et al. Novel histone deacetylase inhibitor AR-42 exhibits antitumor activity in pancreatic cancer cells by affecting multiple biochemical pathways. PLoS One. 2017,12(8):e0183368. doi: 10.1371/journal.pone.0183368Read this paper
- Chen CY, Lin YS, Chen CL, et al. Targeting annexin A2 reduces tumorigenesis and therapeutic resistance of nasopharyngeal carcinoma. Oncotarget. 2015,6(29):26946-59. doi: 10.18632/oncotarget.4521Read this paper
- Wang TH, Lin YS, Chen Y, et al. Long non-coding RNA AOC4P suppresses hepatocellular carcinoma metastasis by enhancing vimentin degradation and inhibiting epithelial-mesenchymal transition. Oncotarget. 2015,6(27):23342-57.Read this paper
- Cheng M, Ho S, Yoo JH, et al. Cathelicidin suppresses colon cancer development by inhibition of cancer associated fibroblasts. Clin Exp Gastroenterol. 2015,8:13-29. doi: 10.2147/CEG.S70906Read this paper
![Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N2C1], Internal (GTX112733) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N2C1], Internal (GTX112733) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX112733/GTX112733_40394_20180316_WB_w_23060500_464.webp)
![Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N2C1], Internal (GTX112733) diluted at 1:1000. Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N2C1], Internal (GTX112733) diluted at 1:1000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX112733/GTX112733_40394_20151105_WB_M_brain_w_23060500_926.webp)

![Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N2C1], Internal (GTX112733) diluted at 1:1000. Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N2C1], Internal (GTX112733) diluted at 1:1000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX112733/GTX112733_40394_20151105_WB_R_brain_w_23060500_519.webp)
![N-Cadherin antibody [N2C1], Internal detects CDH2 protein at membrane on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. N-Cadherin antibody [N2C1], Internal (GTX112733) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min N-Cadherin antibody [N2C1], Internal detects CDH2 protein at membrane on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. N-Cadherin antibody [N2C1], Internal (GTX112733) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX112733/GTX112733_40394_IHC_2_w_23060500_732.webp)
![N-Cadherin antibody [N2C1], Internal detects N-Cadherin protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: N-Cadherin protein stained by N-Cadherin antibody [N2C1], Internal (GTX112733) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). N-Cadherin antibody [N2C1], Internal detects N-Cadherin protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: N-Cadherin protein stained by N-Cadherin antibody [N2C1], Internal (GTX112733) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX112733/GTX112733_40394_20160921_IHC-Fr_w_23060500_134.webp)




![N-Cadherin antibody [N1N3] detects N-Cadherin protein at cell membrane by immunofluorescent analysis. Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: N-Cadherin stained by N-Cadherin antibody [N1N3] (GTX112734) diluted at 1:500. Blue: Hoechst 33342 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX112734/GTX112734_40394_20180704_ICC_IF_w_23060500_124.webp)
![IHC-P analysis of human lung cancer (A), colon cancer (B), ovarian cancer (C) and mammary cancer(D) using GTX82992 N-Cadherin antibody [5D5].](https://www.genetex.com/upload/website/prouct_img/normal/GTX82992/GTX82992_20170912_IHC-P_w_23061322_220.webp)