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N Cadherin Antibody (YA276, Rabbit)

HY-P80761
MedChem Express
TargetCDH2
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    N Cadherin Antibody (YA276, Rabbit)
  • Delivery Days Customer
    10
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID1000
  • Target name
    CDH2
  • Target description
    cadherin 2
  • Target synonyms
    ACOGS, ADHD8, ARVD14, CD325, CDHN, CDw325, NCAD, cadherin-2, N-cadherin 1, cadherin 2, type 1, N-cadherin (neuronal), calcium-dependent adhesion protein, neuronal, neural cadherin
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    N Cadherin Antibody (YA276) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to N Cadherin.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CDH2 using anti-CDH2 antibody (A01577-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human LNCAP whole cell lysates, Lane 2: human SGC-7901 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDH2 antigen affinity purified polyclonal antibody (Catalog # A01577-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDH2 at approximately 140KD. The expected band size for CDH2 is at 100KD.
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