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ProductsBack/N Cadherin (CDH2) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 7E9]
Detection of endogenous N-Cadherin: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab NCAD-7E9 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: HeLa Lane 2: HepG2 Lane 3: HEK293 Lane 4: SH-SY5Y Lane 5: MDCK Lane 6: PC12 Lane 7: CMT 93 Lane 8: Neuro 2A Lane 9: 3T3
Detection of endogenous N-Cadherin: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab NCAD-7E9 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: HeLa Lane 2: HepG2 Lane 3: HEK293 Lane 4: SH-SY5Y Lane 5: MDCK Lane 6: PC12 Lane 7: CMT 93 Lane 8: Neuro 2A Lane 9: 3T3
Detection of endogenous N-Cadherin: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab NCAD-7E9 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: HeLa Lane 2: HepG2 Lane 3: HEK293 Lane 4: SH-SY5Y Lane 5: MDCK Lane 6: PC12 Lane 7: CMT 93 Lane 8: Neuro 2A Lane 9: 3T3

N Cadherin (CDH2) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 7E9]

AM00013PU-N
OriGene
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetCDH2
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    N Cadherin (CDH2) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 7E9]
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    7E9
  • Gene ID1000
  • Target name
    CDH2
  • Target description
    cadherin 2
  • Target synonyms
    ACOGS, ADHD8, ARVD14, CD325, CDHN, CDw325, NCAD, cadherin-2, N-cadherin 1, cadherin 2, type 1, N-cadherin (neuronal), calcium-dependent adhesion protein, neuronal, neural cadherin
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP19022
  • Protein Name
    Cadherin-2
  • Scientific Description
    N Cadherin (CDH2) (incl. pos. control) mouse monoclonal antibody, clone 7E9, Purified
  • Reactivity
    Human, Mouse
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CDH2 using anti-CDH2 antibody (A01577-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human LNCAP whole cell lysates, Lane 2: human SGC-7901 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDH2 antigen affinity purified polyclonal antibody (Catalog # A01577-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDH2 at approximately 140KD. The expected band size for CDH2 is at 100KD.
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