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ProductsBack/Na+ Taurocholate Cotransporting Polypeptide (NTCP) Monoclonal Antibody

Na+ Taurocholate Cotransporting Polypeptide (NTCP) Monoclonal Antibody

CAU32745
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetSLC10A1
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Overview

  • Supplier
    Biomatik
  • Product Name
    Na+ Taurocholate Cotransporting Polypeptide (NTCP) Monoclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    C1
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6554
  • Target name
    SLC10A1
  • Target description
    solute carrier family 10 member 1
  • Target synonyms
    cell growth-inhibiting gene 29 protein; FHCA2; growth-inhibiting protein 29; Na(+)/bile acid cotransporter; Na(+)/taurocholate transport protein; Na/taurocholate cotransporting polypeptide; NTCP; sodium/bile acid cotransporter; sodium/taurocholate cotransporter; sodium/taurocholate cotransporting polypeptide; solute carrier family 10 (sodium/bile acid cotransporter family), member 1; solute carrier family 10 (sodium/bile acid cotransporter), member 1
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDQ14973
  • Protein Name
    Sodium/bile acid cotransporter
  • Scientific Description
    The Na+ Taurocholate Cotransporting Polypeptide (NTCP) Monoclonal Antibody (Species: Human) has been validated for the following applications: WB, IHC, ICC, IP.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SLC10A1/NTCP1 using anti-SLC10A1/NTCP1 antibody (A06872-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey liver tissue lysates, Lane 2: human HCCP tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC10A1/NTCP1 antigen affinity purified polyclonal antibody (Catalog # A06872-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC10A1/NTCP1 at approximately 38 kDa. The expected band size for SLC10A1/NTCP1 is at 38 kDa.
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