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Western Blot analysis of A549 cells using NTCP Polyclonal Antibody.
Western Blot analysis of A549 cells using NTCP Polyclonal Antibody.
Western Blot analysis of A549 cells using NTCP Polyclonal Antibody.

SLC10A1 Antibody

CSB-PA005605
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetSLC10A1
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Overview

  • Supplier
    Cusabio
  • Product Name
    SLC10A1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6554
  • Target name
    SLC10A1
  • Target description
    solute carrier family 10 member 1
  • Target synonyms
    cell growth-inhibiting gene 29 protein; FHCA2; growth-inhibiting protein 29; Na(+)/bile acid cotransporter; Na(+)/taurocholate transport protein; Na/taurocholate cotransporting polypeptide; NTCP; sodium/bile acid cotransporter; sodium/taurocholate cotransporter; sodium/taurocholate cotransporting polypeptide; solute carrier family 10 (sodium/bile acid cotransporter family), member 1; solute carrier family 10 (sodium/bile acid cotransporter), member 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14973
  • Protein Name
    Sodium/bile acid cotransporter
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SLC10A1/NTCP1 using anti-SLC10A1/NTCP1 antibody (A06872-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey liver tissue lysates, Lane 2: human HCCP tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC10A1/NTCP1 antigen affinity purified polyclonal antibody (Catalog # A06872-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC10A1/NTCP1 at approximately 38 kDa. The expected band size for SLC10A1/NTCP1 is at 38 kDa.
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