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Western Blot analysis of HuvEc cells using NARG1 Polyclonal Antibody
Western Blot analysis of HuvEc cells using NARG1 Polyclonal Antibody
Western Blot analysis of HuvEc cells using NARG1 Polyclonal Antibody

NAA15 Antibody

CSB-PA003372
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetNAA15
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Overview

  • Supplier
    Cusabio
  • Product Name
    NAA15 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID80155
  • Target name
    NAA15
  • Target description
    N-alpha-acetyltransferase 15, NatA auxiliary subunit
  • Target synonyms
    Ga19, MRD50, NARG1, NAT1P, NATH, TBDN, TBDN100, N-alpha-acetyltransferase 15, NatA auxiliary subunit, N-terminal acetyltransferase, NMDA receptor regulated 1, NMDA receptor-regulated protein 1, gastric cancer antigen Ga19, protein tubedown-1, transcriptional coactivator tubedown-100, tubedown-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BXJ9
  • Protein Name
    N-alpha-acetyltransferase 15, NatA auxiliary subunit
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of NARG1 using anti-NARG1 antibody (A05636-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: 293T whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NARG1 antigen affinity purified polyclonal antibody (Catalog # A05636-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NARG1 at approximately 101KD. The expected band size for NARG1 is at 101KD.
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