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NARG1 antibody

GTX115560
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetNAA15
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Overview

  • Supplier
    GeneTex
  • Product Name
    NARG1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.66 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID80155
  • Target name
    NAA15
  • Target description
    N-alpha-acetyltransferase 15, NatA auxiliary subunit
  • Target synonyms
    Ga19, MRD50, NARG1, NAT1P, NATH, TBDN, TBDN100, N-alpha-acetyltransferase 15, NatA auxiliary subunit, N-terminal acetyltransferase, NMDA receptor regulated 1, NMDA receptor-regulated protein 1, gastric cancer antigen Ga19, protein tubedown-1, transcriptional coactivator tubedown-100, tubedown-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BXJ9
  • Protein Name
    N-alpha-acetyltransferase 15, NatA auxiliary subunit
  • Scientific Description
    This gene encodes a protein of unknown function. However, similarity to proteins in yeast and other species suggests that this protein may be an N-acetyltransferase. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Sundararaman B, Zhan L, Blue SM, et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 2016,61(6):903-13. doi: 10.1016/j.molcel.2016.02.012
    Read this paper

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Figure 1. Western blot analysis of NARG1 using anti-NARG1 antibody (A05636-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: 293T whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NARG1 antigen affinity purified polyclonal antibody (Catalog # A05636-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NARG1 at approximately 101KD. The expected band size for NARG1 is at 101KD.
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