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F9 cell lysates; probed with Nanog (1A11) Monoclonal Antibody (bsm-52124R) at 1:1000 overnight at 4ËšC. Followed by a conjugated secondary antibody.
F9 cell lysates; probed with Nanog (1A11) Monoclonal Antibody (bsm-52124R) at 1:1000 overnight at 4ËšC. Followed by a conjugated secondary antibody.
F9 cell lysates; probed with Nanog (1A11) Monoclonal Antibody (bsm-52124R) at 1:1000 overnight at 4ËšC. Followed by a conjugated secondary antibody.

Nanog Recombinant Antibody

BSM-52124R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetNANOG
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Overview

  • Supplier
    Bioss
  • Product Name
    Nanog Recombinant Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(WB=1:500-1000), IHC-P(IHC-P=1:100-500), IHC-F(IHC-F=1:50-200), IF(IF=1:50-200), ICC/IF(ICC/IF=1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID79923
  • Target name
    NANOG
  • Target description
    Nanog homeobox
  • Target synonyms
    homeobox protein NANOG, homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H9S0
  • Protein Name
    Homeobox protein NANOG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of Nanog using anti-Nanog antibody (A00153-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat ovary tissue lysates, Lane 2: mouse ovary tissue lysates, Lane 3: MCF-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nanog antigen affinity purified polyclonal antibody (Catalog # A00153-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nanog at approximately 48KD. The expected band size for Nanog is at 34KD.
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