Bio-Connect
F9 cell lysates; probed with Nanog (1A11) Monoclonal Antibody (bsm-52124R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
F9 cell lysates; probed with Nanog (1A11) Monoclonal Antibody (bsm-52124R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
F9 cell lysates; probed with Nanog (1A11) Monoclonal Antibody (bsm-52124R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.

Nanog Recombinant Antibody

BSM-52124R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetNANOG
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Nanog (1A11) Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(WB=1:500-1000), IHC-P(IHC-P=1:100-500), IHC-F(IHC-F=1:50-200), IF(IF=1:50-200), ICC/IF(ICC/IF=1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID79923
  • Target name
    NANOG
  • Target description
    Nanog homeobox
  • Target synonyms
    homeobox protein NANOG; homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H9S0
  • Protein Name
    Homeobox protein NANOG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
Biomatik
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetNANOG
  • SizePrice
Product group Antibodies
Assay Genie
TargetNANOG
  • SizePrice
Product group Antibodies
RayBiotech
ApplicationsELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
MedChem Express
TargetNANOG
  • SizePrice
Western Blot Positive WB detected in: Ntera-2 whole cell lysate All lanes: NANOG antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 35, 33 kDa Observed band size: 35 kDa
Product group Antibodies
Cusabio
NANOG AntibodyCSB-PA888008LA01HU
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetNANOG
  • SizePrice
Immunohistochemical staining of human testis cancer shows strong nuclear immunoreactivity in tumor cells.
Product group Antibodies
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetNANOG
  • SizePrice
Product group Antibodies
Everest Biotech
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetNANOG
  • SizePrice
Product group Antibodies
References
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetNANOG
  • SizePrice
Figure 1. Western blot analysis of Nanog using anti-Nanog antibody (A00153-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat ovary tissue lysates, Lane 2: mouse ovary tissue lysates, Lane 3: MCF-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nanog antigen affinity purified polyclonal antibody (Catalog # A00153-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nanog at approximately 48KD. The expected band size for Nanog is at 34KD.
Product group Antibodies
Boster Bio
Anti-Nanog Antibody Picoband(r)A00153-3-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot
TargetNANOG
  • SizePrice
Anti-Nanog Antibody
Product group Antibodies
Antibodies.com
ApplicationsWestern Blot, ELISA
  • SizePrice