Sample (30 μg of whole cell lysate) A: HeLa nucleus 12% SDS PAGE GTX100863 diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Immunoprecipitation of NANOG protein from NT2D1 whole cell extracts using 5 μg of NANOG antibody [N3C3] (GTX100863). Western blot analysis was performed using NANOG antibody [N3C3] (GTX100863) diluted at 1:500. EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.](https://www.genetex.com/upload/website/prouct_img/normal/GTX100863/GTX100863_40436_IP_w_23060100_114.webp "Immunoprecipitation of NANOG protein from NT2D1 whole cell extracts using 5 μg of NANOG antibody [N3C3] (GTX100863). Western blot analysis was performed using NANOG antibody [N3C3] (GTX100863) diluted at 1:500. EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.")
Sample (30 μg of whole cell lysate) A: HeLa nucleus 12% SDS PAGE GTX100863 diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Nanog antibody [N3C3]
GTX100863
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mammals, Mouse
TargetNANOG
Overview
- SupplierGeneTex
- Product NameNanog antibody [N3C3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IP: 1:100-1:500. ELISA: 1:1000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.5 mg/ml
- ConjugateUnconjugated
- Gene ID79923
- Target nameNANOG
- Target descriptionNanog homeobox
- Target synonymshomeobox protein NANOG, homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48
- HostRabbit
- IsotypeIgG
- Protein IDQ9H9S0
- Protein NameHomeobox protein NANOG
- Scientific DescriptionTranscription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5-TAAT[GT][GT]-3 or 5-[CG][GA][CG]C[GC]ATTAN[GC]-3 (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.
- ReactivityHuman, Mammals, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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