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Western blot All lanes: NAPA antibody at 7microg/ml Lane 1: 293T whole cell lysate Lane 2: Hela whole cell lysate Lane 3: K562 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 34 kDa Observed band size: 34 kDa
Western blot All lanes: NAPA antibody at 7microg/ml Lane 1: 293T whole cell lysate Lane 2: Hela whole cell lysate Lane 3: K562 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 34 kDa Observed band size: 34 kDa
Western blot All lanes: NAPA antibody at 7microg/ml Lane 1: 293T whole cell lysate Lane 2: Hela whole cell lysate Lane 3: K562 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 34 kDa Observed band size: 34 kDa

NAPA Antibody

CSB-PA015447LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetNAPA
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Overview

  • Supplier
    Cusabio
  • Product Name
    NAPA Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8775
  • Target name
    NAPA
  • Target description
    NSF attachment protein alpha
  • Target synonyms
    alpha-SNAP; alpha-soluble NSF attachment protein; N-ethylmaleimide-sensitive factor attachment protein, alpha; SNAPA
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP54920
  • Protein Name
    Alpha-soluble NSF attachment protein
  • Scientific Description
    Required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Alpha SNAP/NAPA using anti-Alpha SNAP/NAPA antibody (A05153-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human THP-1 whole cell lysates, Lane 6: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha SNAP/NAPA antigen affinity purified polyclonal antibody (Catalog # A05153-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alpha SNAP/NAPA at approximately 33 kDa. The expected band size for Alpha SNAP/NAPA is at 33 kDa.
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