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SNAP alpha Antibody (YA2875, Rabbit)

HY-P83130
MedChem Express
TargetNAPA
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    SNAP alpha Antibody (YA2875, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID8775
  • Target name
    NAPA
  • Target description
    NSF attachment protein alpha
  • Target synonyms
    SNAPA, alpha-soluble NSF attachment protein, N-ethylmaleimide-sensitive factor attachment protein, alpha, alpha-SNAP
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    SNAP alpha Antibody (YA2875) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2875), targeting SNAP alpha, with a predicted molecular weight of 33 kDa (observed band size: 33 kDa). SNAP alpha Antibody (YA2875) can be used for WB, IHC-P, ICC/IF experiment in human, mouse, rat background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Alpha SNAP/NAPA using anti-Alpha SNAP/NAPA antibody (A05153-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human THP-1 whole cell lysates, Lane 6: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha SNAP/NAPA antigen affinity purified polyclonal antibody (Catalog # A05153-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alpha SNAP/NAPA at approximately 33 kDa. The expected band size for Alpha SNAP/NAPA is at 33 kDa.
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