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Western Blot analysis of Hela and Jurkat cell, Mouse brain tissue and 293T cell using NAPA Polyclonal Antibody at dilution of 1:500
Western Blot analysis of Hela and Jurkat cell, Mouse brain tissue and 293T cell using NAPA Polyclonal Antibody at dilution of 1:500
Western Blot analysis of Hela and Jurkat cell, Mouse brain tissue and 293T cell using NAPA Polyclonal Antibody at dilution of 1:500

NAPA Polyclonal Antibody

E-AB-10600
Elabscience
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetNAPA
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Overview

  • Supplier
    Elabscience
  • Product Name
    NAPA Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    ELISA WB IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.3 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID8775
  • Target name
    NAPA
  • Target description
    NSF attachment protein alpha
  • Target synonyms
    SNAPA, alpha-soluble NSF attachment protein, N-ethylmaleimide-sensitive factor attachment protein, alpha, alpha-SNAP
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP54920
  • Protein Name
    Alpha-soluble NSF attachment protein
  • Scientific Description
    This gene encodes a member of the soluble NSF attachment protein (SNAP) family. SNAP proteins play a critical role in the docking and fusion of vesicles to target membranes as part of the 20S NSF-SNAP-SNARE complex. The encoded protein plays a role in the completion of membrane fusion by mediating the interaction of N-ethylmaleimide-sensitive factor (NSF) with the vesicle-associated and membrane-associated SNAP receptor (SNARE) complex, and stimulating the ATPase activity of NSF. Alternatively spliced transcript variants have been observed for this gene.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Alpha SNAP/NAPA using anti-Alpha SNAP/NAPA antibody (A05153-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human THP-1 whole cell lysates, Lane 6: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha SNAP/NAPA antigen affinity purified polyclonal antibody (Catalog # A05153-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alpha SNAP/NAPA at approximately 33 kDa. The expected band size for Alpha SNAP/NAPA is at 33 kDa.
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