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ProductsBack/NBN / Nibrin Antibody (aa420-774)

NBN / Nibrin Antibody (aa420-774)

LS-C16850
LifeSpan BioSciences
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetNBN
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NBN / Nibrin Antibody (aa420-774)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Suitable for use in Western Blot, Immunoprecipitation. Western Blot Analysis: 2 ug/ml detects Nibrin/Nbs1 in Jurkat, HeLa nuclear extract, HeLa whole cell, and A431+/-EGF lysates. Included Positive Antigen Control: Jurkat lysate. Add 2.5 ul of 2-mercaptoethanol/100 ul of lysate and boil for 5 minutes to reduce the preparation. Load 20 ug of reduced lysate per lane for minigels. Immunoprecipitation: 4 ug immunoprecipitates Nibrin/Nbs1 from 500 ug of Jurkat RIPA lysate. Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti- Nibrin/Nbs1 (2 ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Nibrin/Nbs1 (95kD).. IP, WB Suitable for use in Western Blot, Immunoprecipitation. Western Blot Analysis: 2 ug/ml detects Nibrin/Nbs1 in Jurkat, HeLa nuclear extract, HeLa whole cell, and A431+/-EGF lysates. Included Positive Antigen Control: Jurkat lysate. Add 2.5 ul of 2-mercaptoethanol/100 ul of lysate and boil for 5 minutes to reduce the preparation. Load 20 ug of reduced lysate per lane for minigels. Immunoprecipitation: 4 ug immunoprecipitates Nibrin/Nbs1 from 500 ug of Jurkat RIPA lysate. Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti- Nibrin/Nbs1 (2 ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Nibrin/Nbs1 (95kD).
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4683
  • Target name
    NBN
  • Target description
    nibrin
  • Target synonyms
    ATV; AT-V1; AT-V2; cell cycle regulatory protein p95; NBS; NBS1; nibrin; Nijmegen breakage syndrome 1 (nibrin); P95; p95 protein of the MRE11/RAD50 complex
  • Host
    Mouse
  • Isotype
    IgG
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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