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Phospho-Nbn (S343) Recombinant Antibody

CAC12468
Biomatik
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetNBN
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Overview

  • Supplier
    Biomatik
  • Product Name
    Phospho-Nbn (S343) Recombinant Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1B4
  • Conjugate
    Unconjugated
  • Gene ID4683
  • Target name
    NBN
  • Target description
    nibrin
  • Target synonyms
    ATV; AT-V1; AT-V2; cell cycle regulatory protein p95; NBS; NBS1; nibrin; Nijmegen breakage syndrome 1 (nibrin); P95; p95 protein of the MRE11/RAD50 complex
  • Isotype
    IgG
  • Protein IDO60934
  • Protein Name
    Nibrin
  • Scientific Description
    The Phospho-Nbn (S343) Recombinant Antibody (Species: Human) has been validated for the following applications: ELISA, WB.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of p95 NBS1 using anti-p95 NBS1 antibody (PB9615). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-p95 NBS1 antigen affinity purified polyclonal antibody (Catalog # PB9615) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for p95 NBS1 at approximately 95 kDa. The expected band size for p95 NBS1 is at 85 kDa.
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GeneTex
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