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Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NBS1 antibody (GTX103229) diluted at 1:2000.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NBS1 antibody (GTX103229) diluted at 1:2000.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NBS1 antibody (GTX103229) diluted at 1:2000.

NBS1 antibody

GTX103229
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetNBN
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Overview

  • Supplier
    GeneTex
  • Product Name
    NBS1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4683
  • Target name
    NBN
  • Target description
    nibrin
  • Target synonyms
    AT-V1, AT-V2, ATV, NBS, NBS1, P95, hNbs1, nibrin, Nijmegen breakage syndrome 1 (nibrin), cell cycle regulatory protein p95, p95 protein of the MRE11/RAD50 complex
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60934
  • Protein Name
    Nibrin
  • Scientific Description
    Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. [provided by RefSeq]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of p95 NBS1 using anti-p95 NBS1 antibody (PB9615). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-p95 NBS1 antigen affinity purified polyclonal antibody (Catalog # PB9615) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for p95 NBS1 at approximately 95 kDa. The expected band size for p95 NBS1 is at 85 kDa.
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