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ProductsBack/NBR1 (4BR): m-IgG1 BP-HRP Bundle

NBR1 (4BR): m-IgG1 BP-HRP Bundle

SC-541730
Santa Cruz Biotechnology
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNBR1
1 kit
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    NBR1 (4BR): m-IgG1 BP-HRP Bundle
  • Delivery Days Customer
    5
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID4077
  • Target name
    NBR1
  • Target description
    NBR1 autophagy cargo receptor
  • Target synonyms
    1A1-3B, IAI3B, M17S2, MIG19, next to BRCA1 gene 1 protein, B-box protein, cell migration-inducing gene 19 protein, membrane component, chromosome 17, surface marker 2 (ovarian carcinoma antigen CA125), migration-inducing protein 19, neighbor of BRCA1 gene 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of NBR1 using anti-NBR1 antibody (A03534-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NBR1 antigen affinity purified polyclonal antibody (Catalog # A03534-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NBR1 at approximately 140 kDa. The expected band size for NBR1 is at 107,140 kDa.
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