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Western Blot analysis of Jk cells using Neurocan Polyclonal Antibody
Western Blot analysis of Jk cells using Neurocan Polyclonal Antibody
Western Blot analysis of Jk cells using Neurocan Polyclonal Antibody

NCAN Antibody

CSB-PA007377
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetNCAN
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Overview

  • Supplier
    Cusabio
  • Product Name
    NCAN Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1463
  • Target name
    NCAN
  • Target description
    neurocan
  • Target synonyms
    CSPG3, neurocan core protein, chondroitin sulfate proteoglycan 3 (neurocan), neurocan proteoglycan
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO14594
  • Protein Name
    Neurocan core protein
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Neurocan using anti-Neurocan antibody (A06700-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Neurocan antigen affinity purified polyclonal antibody (Catalog # A06700-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Neurocan at approximately 200KD. The expected band size for Neurocan is at 143KD.
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