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NCAN / Neurocan Antibody

LS-C832604
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetNCAN
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NCAN / Neurocan Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA (1:5000 - 1:10000), IHC (1:25 - 1:100), WB (1:1000 - 1:5000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.56 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID1463
  • Target name
    NCAN
  • Target description
    neurocan
  • Target synonyms
    CSPG3, neurocan core protein, chondroitin sulfate proteoglycan 3 (neurocan), neurocan proteoglycan
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Neurocan using anti-Neurocan antibody (A06700-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Neurocan antigen affinity purified polyclonal antibody (Catalog # A06700-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Neurocan at approximately 200KD. The expected band size for Neurocan is at 143KD.
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