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ProductsBack/NCF1 Polyclonal Antibody
Mouse bone, lymph node, and spleen lysates probed with Polyclonal Antibody, unconjugated (bs-3886R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.
Mouse bone, lymph node, and spleen lysates probed with Polyclonal Antibody, unconjugated (bs-3886R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.
Mouse bone, lymph node, and spleen lysates probed with Polyclonal Antibody, unconjugated (bs-3886R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.

NCF1 Polyclonal Antibody

BS-3886R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetNCF1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    NCF1/p47 phox Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID653361
  • Target name
    NCF1
  • Target description
    neutrophil cytosolic factor 1
  • Target synonyms
    47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; CGD1; NADPH oxidase organizer 2; NCF-1; NCF1A; NCF-47K; neutrophil cytosol factor 1; neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1); neutrophil NADPH oxidase factor 1; nox organizer 2; NOXO2; nox-organizing protein 2; p47phox; p47-phox; SH3 and PX domain-containing protein 1A; SH3PXD1A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP14598
  • Protein Name
    Neutrophil cytosol factor 1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NCF1 using anti-NCF1 antibody (A01586-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human blood lysates, Lane 3: rat thymus tissue lysates, Lane 4: rat lung tissue lysates, Lane 5: mouse NIH3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCF1 antigen affinity purified polyclonal antibody (Catalog # A01586-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NCF1 at approximately 47KD. The expected band size for NCF1 is at 44KD.
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