ELISA analysis of antigen using GTX60593 NCK1 antibody [5B7]. Black : Control antigen 100ng Purple : Antigen 10ng Blue : Antigen 50ng Red : Antigen 100ng
![FACS analysis of Jurkat cells using GTX60593 NCK1 antibody [5B7]. Green : NCK1 Red : negative control](https://www.genetex.com/upload/website/prouct_img/normal/GTX60593/GTX60593_20170912_FACS_w_23061123_923.webp "FACS analysis of Jurkat cells using GTX60593 NCK1 antibody [5B7]. Green : NCK1 Red : negative control")
![IHC-P analysis of rectum cancer tissue using GTX60593 NCK1 antibody [5B7].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60593/GTX60593_20170912_IHC-P_w_23061123_734.webp "IHC-P analysis of rectum cancer tissue using GTX60593 NCK1 antibody [5B7].")
![WB analysis of Jurkat (1), HeLa (2), HEK293 (3), A431 (4), K562 (5), and COS7 (6) cell lysate using GTX60593 NCK1 antibody [5B7].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60593/GTX60593_20170912_WB_1_w_23061123_779.webp "WB analysis of Jurkat (1), HeLa (2), HEK293 (3), A431 (4), K562 (5), and COS7 (6) cell lysate using GTX60593 NCK1 antibody [5B7].")
![WB analysis of human NCK1 (AA: 203-371) recombinant protein using GTX60593 NCK1 antibody [5B7].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60593/GTX60593_20170912_WB_w_23061123_219.webp "WB analysis of human NCK1 (AA: 203-371) recombinant protein using GTX60593 NCK1 antibody [5B7].")
ELISA analysis of antigen using GTX60593 NCK1 antibody [5B7]. Black : Control antigen 100ng Purple : Antigen 10ng Blue : Antigen 50ng Red : Antigen 100ng
NCK1 antibody [5B7]
GTX60593
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Monkey
TargetNCK1
Overview
- SupplierGeneTex
- Product NameNCK1 antibody [5B7]
- Delivery Days Customer9
- Application Supplier NoteWB: 1/500 - 1/2000. IHC-P: 1/200 - 1/1000. FACS: 1/200 - 1/400. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID5B7
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID4690
- Target nameNCK1
- Target descriptionNCK adaptor protein 1
- Target synonymscytoplasmic protein NCK1; melanoma NCK protein; NCK; NCK tyrosine kinase; nck-1; NCKalpha; non-catalytic region of tyrosine kinase; SH2/SH3 adaptor protein NCK-alpha
- HostMouse
- IsotypeIgG1
- Protein IDP16333
- Protein NameCytoplasmic protein NCK1
- Scientific DescriptionThe protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Jun 2010]
- ReactivityHuman, Monkey
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203