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Western blot All lanes: NCK2 antibody at 10ug/ml Lane 1: Mouse spleen tissue Lane 2: Mouse brain tissue Lane 3: Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 43 kDa Observed band size: 43 kDa
Western blot All lanes: NCK2 antibody at 10ug/ml Lane 1: Mouse spleen tissue Lane 2: Mouse brain tissue Lane 3: Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 43 kDa Observed band size: 43 kDa
Western blot All lanes: NCK2 antibody at 10ug/ml Lane 1: Mouse spleen tissue Lane 2: Mouse brain tissue Lane 3: Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 43 kDa Observed band size: 43 kDa

NCK2 Antibody

CSB-PA015531ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetNCK2
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Overview

  • Supplier
    Cusabio
  • Product Name
    NCK2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8440
  • Target name
    NCK2
  • Target description
    NCK adaptor protein 2
  • Target synonyms
    cytoplasmic protein NCK2; GRB4; growth factor receptor-bound protein 4; NCKbeta; noncatalytic region of tyrosine kinase, beta; SH2/SH3 adaptor protein NCK-beta
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43639
  • Protein Name
    Cytoplasmic protein NCK2
  • Scientific Description
    Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NCK2 using anti-NCK2 antibody (M04036-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCK2 antigen affinity purified monoclonal antibody (Catalog # M04036-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NCK2 at approximately 43 kDa. The expected band size for NCK2 is at 43 kDa.
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