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NCK2 Antibody (YA3293, Rabbit)

HY-P83548
MedChem Express
TargetNCK2
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    NCK2 Antibody (YA3293, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID8440
  • Target name
    NCK2
  • Target description
    NCK adaptor protein 2
  • Target synonyms
    cytoplasmic protein NCK2; GRB4; growth factor receptor-bound protein 4; NCKbeta; noncatalytic region of tyrosine kinase, beta; SH2/SH3 adaptor protein NCK-beta
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    NCK2 Antibody (YA3293) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA3293), targeting NCK2, with a predicted molecular weight of 43 kDa (observed band size: 43 kDa). NCK2 Antibody (YA3293) can be used for WB, ICC/IF experiment in human, mouse, rat background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NCK2 using anti-NCK2 antibody (M04036-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCK2 antigen affinity purified monoclonal antibody (Catalog # M04036-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NCK2 at approximately 43 kDa. The expected band size for NCK2 is at 43 kDa.
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