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ProductsBack/NCLN Antibody (aa375-425)

NCLN Antibody (aa375-425)

LS-C763133
LifeSpan BioSciences
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetNCLN
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NCLN Antibody (aa375-425)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Immunoprecipitation Range: 50 microl/mg lysate
  • Applications
    ImmunoPrecipitation, Western Blot
  • Applications Supplier
    IP, WB (1:1000) Immunoprecipitation Range: 50 µl/mg lysate
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID56926
  • Target name
    NCLN
  • Target description
    nicalin
  • Target synonyms
    NET59; nicalin; nicalin homolog; nicastrin-like protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NCLN using anti-NCLN antibody (A12284-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CCRF-CEM whole cell lysates, Lane 2: human HUH-7 whole cell lysates, Lane 3: rat skeletal muscle tissue lysates, Lane 4: rat L6 whole cell lysates, Lane 5: mouse pancreas tissue lysates, Lane 6: mouse skeletal muscle tissue lysates, Lane 7: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCLN antigen affinity purified polyclonal antibody (Catalog # A12284-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NCLN at approximately 63 kDa. The expected band size for NCLN is at 63 kDa.
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