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NCOA5 Antibody

CSB-PA040101
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetNCOA5
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Overview

  • Supplier
    Cusabio
  • Product Name
    NCOA5 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID57727
  • Target name
    NCOA5
  • Target description
    nuclear receptor coactivator 5
  • Target synonyms
    CIA, bA465L10.6, nuclear receptor coactivator 5, NCoA-5, coactivator independent of AF-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9HCD5
  • Protein Name
    Nuclear receptor coactivator 5
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NCOA5 using anti-NCOA5 antibody (A07810-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCOA5 antigen affinity purified polyclonal antibody (Catalog # A07810-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NCOA5 at approximately 66 kDa. The expected band size for NCOA5 is at 66 kDa.
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