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NCSTN Polyclonal Antibody

RD76014A
Reddot Biotech
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetNCSTN
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Overview

  • Supplier
    Reddot Biotech
  • Product Name
    NCSTN Polyclonal Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.4 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID23385
  • Target name
    NCSTN
  • Target description
    nicastrin
  • Target synonyms
    ATAG1874, nicastrin, anterior pharynx-defective 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    This gene encodes a type I transmembrane glycoprotein that is an integral component of the multimeric gamma-secretase complex. The encoded protein cleaves integral membrane proteins, including Notch receptors and beta-amyloid precursor protein, and may be a stabilizing cofactor required for gamma-secretase complex assembly.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Nicastrin/NCSTN using anti-Nicastrin/NCSTN antibody (A03144-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: rat pancreas tissue lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nicastrin/NCSTN antigen affinity purified polyclonal antibody (Catalog # A03144-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nicastrin/NCSTN at approximately 120 kDa. The expected band size for Nicastrin/NCSTN is at 120 kDa.
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