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NDRG1 Polyclonal Antibody

CAC15826
Biomatik
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetNDRG1
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Overview

  • Supplier
    Biomatik
  • Product Name
    NDRG1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10397
  • Target name
    NDRG1
  • Target description
    N-myc downstream regulated 1
  • Target synonyms
    CAP43, CMT4D, DRG-1, DRG1, GC4, HMSNL, NDR1, NMSL, PROXY1, RIT42, RTP, TARG1, TDD5, protein NDRG1, N-myc downstream-regulated gene 1 protein, differentiation-related gene 1 protein, nickel-specific induction protein Cap43, protein regulated by oxygen-1, reducing agents and tunicamycin-responsive protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92597
  • Protein Name
    Protein NDRG1
  • Scientific Description
    The NDRG1 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC, IF.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NDRG1 using anti-NDRG1 antibody (A01327-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human U-87MG whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDRG1 antigen affinity purified polyclonal antibody (Catalog # A01327-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDRG1 at approximately 46KD. The expected band size for NDRG1 is at 46KD.
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