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Western Blot analysis of Human lymphoma tissue using NDRG1 Polyclonal Antibody at dilution of 1:1000
Western Blot analysis of Human lymphoma tissue using NDRG1 Polyclonal Antibody at dilution of 1:1000
Western Blot analysis of Human lymphoma tissue using NDRG1 Polyclonal Antibody at dilution of 1:1000

NDRG1 Polyclonal Antibody

E-AB-15993
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    NDRG1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA WB IHC
  • Certification
    Research Use Only
  • Concentration
    0.6mg/ml
  • Scientific Description
    This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. The encoded protein is necessary for p53-mediated caspase activation and apoptosis. Mutations in this gene are a cause of Charcot-Marie-Tooth disease type 4D, and expression of this gene may be a prognostic indicator for several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NDRG1 using anti-NDRG1 antibody (A01327-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human U-87MG whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDRG1 antigen affinity purified polyclonal antibody (Catalog # A01327-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDRG1 at approximately 46KD. The expected band size for NDRG1 is at 46KD.
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