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The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA579687(NDUFA5 Antibody) at dilution 1/60, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA579687(NDUFA5 Antibody) at dilution 1/60, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA579687(NDUFA5 Antibody) at dilution 1/60, on the right is treated with fusion protein. (Original magnification: x200)

NDUFA5 Antibody

CSB-PA579687
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNDUFA5
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Overview

  • Supplier
    Cusabio
  • Product Name
    NDUFA5 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4698
  • Target name
    NDUFA5
  • Target description
    NADH:ubiquinone oxidoreductase subunit A5
  • Target synonyms
    B13, CI-13KD-B, CI-13kB, NUFM, UQOR13, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa, NADH-ubiquinone oxidoreductase 13 kDa-B subunit, complex I 13kDa subunit B, complex I subunit B13, type I dehydrogenase, ubiquinone reductase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16718
  • Protein Name
    NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5
  • Scientific Description
    This nuclear gene encodes a conserved protein that comprises the B13 subunit of complex I of the mitochondrial respiratory chain. The encoded protein localizes to the inner mitochondrial membrane, where it is thought to aid in the transfer of electrons from NADH to ubiquinone. Alternative splicing results in multiple transcript variants. There are numerous pseudogenes of this gene on chromosomes 1, 3, 6, 8, 9, 11, 12, and 16.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NDUFA5 using anti-NDUFA5 antibody (A09003-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: rat heart tissue lysates, Lane 4: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFA5 antigen affinity purified polyclonal antibody (Catalog # A09003-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFA5 at approximately 16 kDa. The expected band size for NDUFA5 is at 13 kDa.
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