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Anti-NDUFA5 Antibody

CAB3976
Assay Genie
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetNDUFA5
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Overview

  • Supplier
    Assay Genie
  • Product Name
    Anti-NDUFA5 Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB,IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    Not applicable
  • Conjugate
    Unconjugated
  • Gene ID4698
  • Target name
    NDUFA5
  • Target description
    NADH:ubiquinone oxidoreductase subunit A5
  • Target synonyms
    B13, CI-13KD-B, CI-13kB, NUFM, UQOR13, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa, NADH-ubiquinone oxidoreductase 13 kDa-B subunit, complex I 13kDa subunit B, complex I subunit B13, type I dehydrogenase, ubiquinone reductase
  • Host
    Rabbit
  • Protein IDQ16718
  • Protein Name
    NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5
  • Scientific Description
    NDUFA5 Antibody is a premium polyclonal that offers outstanding performance and reliability for demanding research applications. Rigorously validated for WB, ELISA, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Human,Mouse,Rat samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 100microL format to meet your experimental needs. For optimal performance, store at -20°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Human
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NDUFA5 using anti-NDUFA5 antibody (A09003-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: rat heart tissue lysates, Lane 4: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFA5 antigen affinity purified polyclonal antibody (Catalog # A09003-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFA5 at approximately 16 kDa. The expected band size for NDUFA5 is at 13 kDa.
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Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NDUFA5 Polyclonal Antibody, Unconjugated (bs-55143R) at 1:100 overnight at 4°C, DAB staining.
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