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NDUFA7 Antibody

LS-C668331
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetNDUFA7
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NDUFA7 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Western blot analysis of NDUFA7 expression in HepG2 (A), MCF7 (B), mouse brain (C), rat liver (D) whole cell lysates. Immunohistochemical analysis of NDUFA7 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.. IHC (1:50 - 1:200), IHC-P, WB (1:500 - 1:2000) Western blot analysis of NDUFA7 expression in HepG2 (A), MCF7 (B), mouse brain (C), rat liver (D) whole cell lysates. Immunohistochemical analysis of NDUFA7 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4701
  • Target name
    NDUFA7
  • Target description
    NADH:ubiquinone oxidoreductase subunit A7
  • Target synonyms
    B14.5a; CI-B14.5a; complex I B14.5a subunit; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 7, 14.5kDa; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7; NADH-ubiquinone oxidoreductase subunit B14.5a
  • Host
    Rabbit
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NDUFA7 using anti-NDUFA7 antibody (A10817-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFA7 antigen affinity purified polyclonal antibody (Catalog # A10817-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFA7 at approximately 15 kDa. The expected band size for NDUFA7 is at 13 kDa.
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