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Paraformaldehyde-fixed, paraffin embedded Human liver damage; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NDUFA7 Polyclonal Antibody, Unconjugated (bs-55144R) at 1:100 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human liver damage; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NDUFA7 Polyclonal Antibody, Unconjugated (bs-55144R) at 1:100 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human liver damage; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NDUFA7 Polyclonal Antibody, Unconjugated (bs-55144R) at 1:100 overnight at 4°C, DAB staining.

NDUFA7 Polyclonal Antibody

BS-55144R
Bioss
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNDUFA7
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Overview

  • Supplier
    Bioss
  • Product Name
    NDUFA7 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IHC()
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID4701
  • Target name
    NDUFA7
  • Target description
    NADH:ubiquinone oxidoreductase subunit A7
  • Target synonyms
    B14.5a, CI-B14.5a, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 7, 14.5kDa, NADH-ubiquinone oxidoreductase subunit B14.5a, complex I B14.5a subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95182
  • Protein Name
    NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NDUFA7 using anti-NDUFA7 antibody (A10817-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFA7 antigen affinity purified polyclonal antibody (Catalog # A10817-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFA7 at approximately 15 kDa. The expected band size for NDUFA7 is at 13 kDa.
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