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NDUFA9 Polyclonal Antibody

BS-8301R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetNDUFA9
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Overview

  • Supplier
    Bioss
  • Product Name
    NDUFA9 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID4704
  • Target name
    NDUFA9
  • Target description
    NADH:ubiquinone oxidoreductase subunit A9
  • Target synonyms
    CC6, CI-39k, CI39k, COQ11, MC1DN26, NDUFS2L, SDR22E1, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial, CI-39kD, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 9, 39kDa, NADH dehydrogenase (ubiquinone) Fe-S protein 2-like (NADH-coenzyme Q reductase), NADH-ubiquinone oxidoreductase 39 kDa subunit, complex I 39kDa subunit, short chain dehydrogenase/reductase family 22E, member 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16795
  • Protein Name
    NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of NDUFA9 using anti-NDUFA9 antibody (A07420-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: rat RH35 whole cell lysates, Lane 4: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFA9 antigen affinity purified polyclonal antibody (Catalog # A07420-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFA9 at approximately 36 kDa. The expected band size for NDUFA9 is at 43 kDa.
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