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Anti-NDUFA9 Antibody

CAB3196
Assay Genie
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetNDUFA9
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Overview

  • Supplier
    Assay Genie
  • Product Name
    Anti-NDUFA9 Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    Not applicable
  • Conjugate
    Unconjugated
  • Gene ID4704
  • Target name
    NDUFA9
  • Target description
    NADH:ubiquinone oxidoreductase subunit A9
  • Target synonyms
    CC6, CI-39k, CI39k, COQ11, MC1DN26, NDUFS2L, SDR22E1, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial, CI-39kD, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 9, 39kDa, NADH dehydrogenase (ubiquinone) Fe-S protein 2-like (NADH-coenzyme Q reductase), NADH-ubiquinone oxidoreductase 39 kDa subunit, complex I 39kDa subunit, short chain dehydrogenase/reductase family 22E, member 1
  • Host
    Rabbit
  • Protein IDQ16795
  • Protein Name
    NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial
  • Scientific Description
    NDUFA9 Antibody is a premium polyclonal that offers outstanding performance and reliability for demanding research applications. Rigorously validated for WB, ELISA, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Human,Mouse,Rat samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 100microL format to meet your experimental needs. For optimal performance, store at -20°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Human
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NDUFA9 using anti-NDUFA9 antibody (A07420-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: rat RH35 whole cell lysates, Lane 4: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFA9 antigen affinity purified polyclonal antibody (Catalog # A07420-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFA9 at approximately 36 kDa. The expected band size for NDUFA9 is at 43 kDa.
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