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ProductsBack/NDUFAF1 Recombinant Antibody, AbBy Fluor-647 Conjugated

NDUFAF1 Recombinant Antibody, AbBy Fluor-647 Conjugated

BSM-62045R-BF647
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetNDUFAF1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    NDUFAF1 Recombinant Antibody, AbBy Fluor-647 Conjugated
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Applications Supplier
    WB(1:300-5000), FCM(1:20-100), IF(ICC)(1:50-200), IF()
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Other Conjugate
  • Gene ID51103
  • Target name
    NDUFAF1
  • Target description
    NADH:ubiquinone oxidoreductase complex assembly factor 1
  • Target synonyms
    CGI65; CGI-65; CIA30; complex I intermediate-associated protein 30, mitochondrial; MC1DN11; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 1; NADH dehydrogenase (ubiquinone) complex I, assembly factor 1; NADH-ubiquinone oxidoreductase 1 alpha subcomplex, assembly factor 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y375
  • Protein Name
    Complex I intermediate-associated protein 30, mitochondrial
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NDUFAF1 using anti-NDUFAF1 antibody (A10145). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFAF1 antigen affinity purified polyclonal antibody (Catalog # A10145) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFAF1 at approximately 36 kDa. The expected band size for NDUFAF1 is at 38 kDa.
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