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Western blot analysis of extracts of various cell lines using NDUFB2 Polyclonal Antibody at 1:3000 dilution.
Western blot analysis of extracts of various cell lines using NDUFB2 Polyclonal Antibody at 1:3000 dilution.
Western blot analysis of extracts of various cell lines using NDUFB2 Polyclonal Antibody at 1:3000 dilution.

NDUFB2 Polyclonal Antibody

E-AB-65459
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    NDUFB2 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. This protein has NADH dehydrogenase activity and oxidoreductase activity. It plays a important role in transfering electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Hydropathy analysis revealed that this subunit and 4 other subunits have an overall hydrophilic pattern, even though they are found within the hydrophobic protein (HP) fraction of complex I.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NDUFB2 using anti-NDUFB2 antibody (A13719-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEPG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFB2 antigen affinity purified polyclonal antibody (Catalog # A13719-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFB2 at approximately 15KD. The expected band size for NDUFB2 is at 15KD.
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