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Western blot analysis of extracts of various cell lines using NDUFB4 Polyclonal Antibody at dilution of 1:3000.
Western blot analysis of extracts of various cell lines using NDUFB4 Polyclonal Antibody at dilution of 1:3000.
Western blot analysis of extracts of various cell lines using NDUFB4 Polyclonal Antibody at dilution of 1:3000.

NDUFB4 Polyclonal Antibody

E-AB-64835
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    NDUFB4 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    This gene encodes a non-catalytic subunit of the multisubunit NADH:ubiquinone oxidoreductase, the first enzyme complex in the mitochondrial electron transport chain (complex I). Mammalian complex I is composed of 45 different subunits and transfers electrons from NADH to ubiquinone.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NDUFB4 using anti-NDUFB4 antibody (A11016-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: rat heart tissue lysates, Lane 3: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFB4 antigen affinity purified polyclonal antibody (Catalog # A11016-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFB4 at approximately 15 kDa. The expected band size for NDUFB4 is at 15 kDa.
Product group Antibodies
Boster Bio
Anti-NDUFB4 Antibody Picoband(r)A11016-1-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetNDUFB4
  • SizePrice
Product group Antibodies
RayBiotech
Anti-NDUFB4 Antibody144-60491
ApplicationsWestern Blot
ReactivityHuman, Mouse
TargetNDUFB4
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L929 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (NDUFB4) polyclonal Antibody, Unconjugated (bs-55145R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Product group Antibodies
Bioss
NDUFB4 Polyclonal AntibodyBS-55145R
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetNDUFB4
  • SizePrice
Immunohistochemistry analysis in human heart muscle and pancreas tissues using Anti-NDUFB4 antibody. Corresponding NDUFB4 RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
Anti-NDUFB4 AntibodyHPA051739
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TargetNDUFB4
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Product group Antibodies
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NDUFB4 Antibody (YA1338, Rabbit)HY-P81593
TargetNDUFB4
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NDUFB4 antibody detects NDUFB4 protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. NDUFB4 antibody (GTX111026) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
Product group Antibodies
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NDUFB4 antibodyGTX111026
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Rat
TargetNDUFB4
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Product group Antibodies
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NDUFB4 / B15 AntibodyLS-C748843
ApplicationsWestern Blot
ReactivityHuman, Mouse
TargetNDUFB4
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Product group Antibodies
Antibodies.com
Anti-NDUFB4 AntibodyA92828
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
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